Selection of Reference Gene for Expression Studies in the Ovary and Pituitary of Spotted Scat (Scatophagus argus) at Different Ovarian Stages

The spotted scat (Scatophagus argus) is the most widely commercially cultured fish in eastern and southern Asia that possess potential economic value. However, up to date, there are no studies reported on selection of suitable reference genes this fish, which crucial for guaranteeing accurate reliable results RT-qPCR analyses. This study aimed at screening stable ovary pituitary different ovarian stages, great significance further research reproductive regulation mechanism female S. argus. In study, we selected twenty-one as candidate genes, including four (EEF1A, ACTB, B2M, HPRT1), seventeen (B2M, APOA1, CGBA, RPLP0, GNRHR, GUSB, TBP, SLC25A5, RPL4, PLA1A, GAPDH, GNB2L1, CTFS, HPRT1, RPS2, TFRC, TUBB4B). To analyze stability first used commonly software, BestKeeper, GeNorm, NormFinder, ΔCt, then established comprehensive rankings with ReFinder. indicate RPL4 EEF1A HPRT1 were appropriate argus, respectively, three developmental stages ovary. Additionally, GeNorm analysis suggested normalization gene expression, two best our knowledge understanding, identify analyzing expression Consequently, can be a foundation future reproduction